This is an experiment showing the application of affinity chromatography to the separation of albumin from horse serum. A brief introduction of affinity chromatography and how it is being used in this specific experiment is given. This appears to be a good experiment to show the advantages of affinity chromatography in separating specific proteins from a complex matrix and would be useful in a biochemistry course or a course that is specifically looking at differing types of chromatography.
Affinity Chromatography
About the Author
Chris Harrison
Christopher R. Harrison, PhD, is an Associate Professor and Senate Distinguished Professor of Chemistry at San Diego State University. His research focuses on analytical chemistry, particularly the development of new…
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