Analysis of Uric Acid Metabolites in Urine (or in synthetic urine) by HPLC-MS

 

Introduction:

This laboratory experiment makes use of HPLC-MS to identify and quantify uric acid, 6-aminouracil (6-AU), and allantoin in synthetic urine, with tyrosine as an internal standard. It is designed for use in Quantitative Analysis laboratory, but may find applications in forensic and biochemistry laboratories as well. An Excel file is provided that serves as a guide to calculations following the experiment.

Analysis (PDF)

Report (Excel)

Developed by:

Dr. Maria Matyska-Pesek

About the Author

ASDL Contributor

Chris Harrison

San Diego State University

Christopher R. Harrison, PhD, is an Associate Professor and Senate Distinguished Professor of Chemistry at San Diego State University. His research focuses on analytical chemistry, particularly the development of new…
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