Reverse Phase HPLC Basics for LC/MS

This site provides a tutorial on practical reverse phase chromatography for LC-MS. Focusing on peptide and protein analysis, Andrew Guzzetta offers appropriate tips on mobile phase gradients, preparing your reversed-phase HPLC column for the first run of the day, and taking care of your column after the day’s separations are complete. The site also provides a representative list of web-based resources on LC.

The website is available here .

About the Author

ASDL Contributor

Chris Harrison

San Diego State University

Christopher R. Harrison, PhD, is an Associate Professor and Senate Distinguished Professor of Chemistry at San Diego State University. His research focuses on analytical chemistry, particularly the development of new…
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