Kinetic Determination of Ascorbic Acid
One method for determining the concentration of glucose in fruit juices is to take advantage of its enzymatic oxidation in the presence of glucose oxidase: β-D-glucose + O2 + H2O → δ-D-gluconolactone […]
Kinetic Method
Kinetic Method for Manganese
Trace levels of manganese can be determined by measuring its catalytic effect on the oxidation of diethylamine by sodium periodate. The rate of formation of the oxidation product is followed spectrophotometrically. A […]
Standardization of a Kinetic Method of Analysis
Shown below is a plot of initial rate vs. substrate concentration for an enzyme-substrate reaction that follows Michaelis-Menton kinetics. An analyst developing a quantitative method for the substrate wishes to use a […]