A series of collaborative learning activities and accompanying text that develop the field of separation science, with a particular emphasis on chromatographic separations, is provided. These activities are intended to be done […]
b. HPLC Retention Order
Students will learn to:
Predict elution order of compounds
Calculate retention factor and selectivity factor
Explain the effect of mobile phase composition on resolution and peak shape at a molecular level
Use elution gradients to improve separation of compounds in a mixture
Explain the effect of flow rate on separation at a molecular level
Students will learn to:
Identify the chemical/physical property that controls separation of compounds in a given scenario
Relate the concept of partition coefficients to the separation of compounds on a column
Predict elution order in separations of compounds in liquid chromatography
Students explore aspects of bonded-phase liquid chromatography including the nature of possible stationary phases and variables that affect retention order. There is an associated text that explains the answers to the questions on the worksheet.