Introduction A series of collaborative learning activities and accompanying text that develop the concept of concentration calibration, utilizing the external standard, internal standard, and standard addition methods. The module is based primarily […]
04. Standardizing Analytical Methods
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A variety of common standardization methods are developed in this activity.
Students explore calculations of the concentration on an unknown under a variety of different conditions and standardization methods.
Students explore a variety of calibration procedures when completing this activity.
Students learn to:
Identify different methods to calibrate instrumentation or analytical methodologies
Choose proper calibration method depending on sample characteristics or instrument.
Describe how standards and/or samples are prepared for each calibration method.
Calculate concentration from information obtained from each calibration method
Students explore a variety of calibration methods when completing this activity.
Students learn to:
explain the differences between external standard and standard addition calibration.
identify when the use of an internal standard is necessary.
discuss the merits and drawbacks of each type of calibration.
calculate the concentration of an analyte given raw data using any combination of calibration methods (external standard, standard addition, and/or internal standard).
develop an appropriate calibration method when faced with a real world analytical problem.
Students explore a variety of calibration methods when completing this activity.
Students learn to:
Explain the purpose of using internal standards
Use response factors to calculate an unknown concentration based on an internal standard
Determine the limits of detection (LOD) and quantification of a method.
Students explore the use of internal standards and standard addition when completing this activity.
Students examine several examples of analyses using standard addition when completing this activity.